Impact Of Nutritional Factors In Microsporum canis Using Response Surface Methodology

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Mansoura University, 35516 Mansoura City, Egypt

2 Plant Pathology Research Institute-Agriculture Research center-Giza-Egypt

Abstract

The three genera of dermatophytes —Trichophyton, Microsporum, and Epidermatophyton—are a group of keratinophilic fungi that can penetrate keratinized tissue and result in dermatophytosis. One of the most casual agents of tinea peds, tinea corporis, and tinea capitates is Microsporum canis. The present inquiry aimed to isolate and identify Microsporum canis at the Dermatology and Venereology Hospital in Mansoura and evaluate the optimal growth medium required for the sporulation and maximum biomass production of the isolated fungi. Microsporum canis was identified morphologically by conventional methods and the effects of various nutritional factors on biomass production were investigated. The significance of eleven independent variables given was investigated by PB design with a set of twenty experiments using different combinations of factors, the impact on fungal growth represented by peptone, yeast extract, maltose, and fructose, with P-value 0.00, followed by Response Surface Methodology using CCD. CCD was employed to assess and optimize the ideal conditions of the four independent variables and the interactions among input variables. The findings of the 31 run trials demonstrated that the yield varied significantly per fungus with the four variables. The maximum biomass was accomplished at run 8 with peptone 15 g/l, yeast extracts 15 g/l, maltose 10 g/l, and fructose 10 g/l with a yield of 19.480 g/l.

Keywords

Mansoura Journal of Biology
Volume 72, Issue 2
June 2025
Pages 57-67

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