The Effect Of Ethanolic Extract From Purslane Leaves on Pathogenic Bacteria Isolated from Liver Transplantation Patients

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt

2 Department of General Surgery,Gastrointetinal surgery center, Faculty of Medicine, Mansoura University,Mansoura,Egypt.

Abstract

Portulaca oleracea is a herbaceous weed belongs to the family of Portulacaceae..Protein from ethanolic extract of  purslane  leaves used instead of immunosuppressive drugs taken by liver transplantation patients.Firstly we prepared ethanolic extract of purslane leaves to study the effect of the alcoholic extract on isolated bacteria as Klebsiella species and Staphylococcus Aureus in wound samples and the lowest percentage of species was Pseudomonas species and Proteus species then SDS-PAGE technique and electroelution by migration of proteins out of gel slices into solution  and determinion of minimum inhibitory concentration (MIC) of electroeluted protein extract and finally Susceptibility test for bacterial pathogens . Objective: The current paper's objective is to study the effect of ethanolic extract from purslane leaves on pathogenic bacteria isolated from liver transplantation patients. Results: For Staphylococcus aureus,Meropenem and Amikacin were reported as the maximum inhibition zone of 27mm.For Proteus sp. the maximum inhibition zone  of 30mm was demonstrated Mikacin and Gentamicin.For Pseudomonas sp. Meropenem was recorded maximum inhibition zone of  30mm. The minimum inhibition concentration(MIC) effect for klebsiella, pseudomonas, and Proteus were at concentration of 0.25 g/ml and for staphylococcus aureus it was at concentration  of 0.35 g/ml

Keywords

Mansoura Journal of Biology
Volume 69, Issue 2
June 2024
Pages 27-34

Files

Share

How to cite

Statistics