Overexpression of fungal laccase enzymes and their application in waste treatment

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt

2 2Microbial Biotechnology Departement, Gentic Engeeniring and Biotechnology Research institute, University of Sadat City,Menofyia governerate, Egypt.

Abstract

Phenolic compounds like phenol, 2,4-dichlorophenol, bisphenol A, 2,4-dinitrophenol, 4-chlorophenol, besides 4-nitrophenol are well-known to be extremely harmful to humans and living organisms. Therefore, it is important to develop appropriate remediation techniques to remove these phenolic compounds effectively from industrial effluents. Biodegradation using enzymatic technology is investigated in detail as it is a promising biotechnological solution to sustainable combat of water pollution caused by phenolic compounds as part of a defined environmentally optimized strategy need to do it.
Objective: The current paper's objective is to study production  of laccases enzyme from white rots fungi and use of immobilized laccases to degrade phenolic compounds in dyes.
Results: Among tested fungal isolates, only onefungus., Pleurotus sp. was showed a highly laccase enzyme productivity,wherein the optimization process for laccases productivity showed to be necessiate .It  is  showed that pH 7.0 and temperature 30c were the bestphysical conditions for optimization of enzyme.As well as,, carbon source such as glucose and  sucrose ,  , nitrogen source(i.e.,  urea and ammonium chloride mixtures) were crucial  nutritional sources affected on the productivity of laccase enzymes. . At molecular biology level, gene coding for laccase was partially estimated by 831bps, and its sequence analysis showed 98.44% with Pleurotus ostreatus,s oxidoreductase (MIA40). Further, laccase protein was estimated by 55- 57KDa.Laccase enzyme and it exhibits activity against phenolic compounds when(revised these sentences) (Azo Dye from Egyptian German For Pigments & Resins) used as a substrate for the enzyme, total phenolic compounds concentration in untreated substrate was 476.4 µg/ml. there was a remarkable reduction in this concentration when laccase enzyme applied to Azo dye as substrate, it showed 127.2 (26.7%) µg/ml

Keywords

Mansoura Journal of Biology
Volume 71, Issue 4
December 2024
Pages 63-74

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