The efficacy of some fungi in Bio-synthesis of silver nanoparticles and characterization through physical and microscopic approaches

Document Type : Original Article

Authors

1 Botany Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt

2 Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, Alexandria, Egypt.

Abstract

Green techniques for the production of metallic nanoparticles have emerged as a new and promising area of research in recent years. Several microbes produce metal nanoparticles that can be extracellular or intracellular and vary in efficiency, size, and form. In this investigation, five fungal mycelial filtrates (Penicillium spp., Alternaria spp., Fusarium spp., Aspergillus spp.  and Trichoderma spp.) were screened for the creation of silver nanoparticles (AgNPs). By examining the fungus filtrate optically only three fungal isolates (Fusarium spp., Aspergillus spp.  and Trichoderma spp.) were best able to reduce the silver salt (AgNO3) into silver nanoparticles. The UV-visible spectra of the biosynthesized nanoparticles (AgNPs) by Fusarium oxysporum cell filtrate showed characteristic surface plasmon absorption at 432 nm. Fourier Transform Infrared (FTIR) analysis of AgNPs synthesized using F. oxysporum showed vibrational frequencies at 3449, 1630, 1025 and 535 cm−1. A Transmission electron microscopy (TEM) image demonstrated the production of spherical AgNPs ranging in size from 5.36 to 20.11 nm in diameter. The green biosynthesis process used in this study is a non-toxic alternative to standard chemical and physical approaches, and it would be suitable for biological large-scale manufacturing and potential therapies.

Keywords

Mansoura Journal of Biology
Volume 62, Issue 1
March 2023
Pages 7-15

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