Antibacterial activity of nanosilver against Escherichia coli and Pseudomonas aeruginosa

Document Type : Original Article

Authors

1 Botany Department, Faculty of Science, Mansoura University, Egypt

2 Botany Department, Faculty of Science, Banha University, Egypt

Abstract

Multidrug resistance bacteria are a major health concern so new strategies for controlling bacterial activity such as nanoparticles are urgently needed. Fusarium oxysporum-based biological approaches for nanoparticle synthesis have been proposed as promisingly green processes. By using UV-visible spectroscopy, which demonstrated a maximum absorption at 434 nm, biosynthesized silver nanoparticles (AgNPs) were characterized. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) techniques showed that monodispersed spherical shapes with mean diameters of 6-50 nm were formed and zeta potential analysis displayed (-16.7) mV with a single. The MIC of  biosynthesized AgNPs for each bacteria (Escherichia coli and Pseudomonas aeruginosa) was determined. The concentration of 10 µg/ml of nano-silver completely inhibited the growth of all tested bacteria. The antibacterial effect of AgNPs on E. coli and P. aeruginosa showed a great potential effect of AgNPs than Ceftriaxone, while an increase of the inhibition zone diameter in the case of AgNPs antibiotic mixture had been investigated.
In conclusion, future therapeutic mechanism for multi-drug resistant pathogenic bacterial  have a lot of potential for using silver nanoparticles as an efficient antibacterial agent.

Keywords

Mansoura Journal of Biology
Volume 62, Issue 1
March 2023
Pages 35-44

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