Isolation and identification of Fusarium oxysporum, the causal fungus of wilt disease of tomato (Solanum lycopersicum)

Document Type : Original Article

Authors

Botany Department, Faculty of Science, Mansoura University, Egypt

Abstract

F. oxysporum is a fungal plant disease that causes tomato plant losses and severe wilting. This pathogen causes widespread diseases in host plants such as crown rot and wilting. The goal of this study was to isolate F. oxysporum, a fungus that causes wilt disease. Plant and soil samples were obtained from several Mansoura University sites. Isolates of F. oxysporum were obtained from soil, tomato roots, tomato seeds, and potato tubers. The pathogen isolates were identified using microscopic and morphological analysis. F. oxysporum morphological identification on growth media was accomplished by describing the oval to kidney/ellipsoid shaped oval tapering, white cottony mycelium with dark-purple undersurface, and 3 septate spores. According to their morphological and microscopic characterization, the isolates were confirmed as F. oxysporum. The purpose of this study was to isolate and determine the morphological characteristics of fungal isolates from F. oxysporum f. sp. lycopersici, the causative agent of tomato wilt disease. Pathogenicity testing is used to select the most aggressive isolate.

Keywords

Mansoura Journal of Biology
Volume 62, Issue 1
March 2023
Pages 58-63

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