Nutritional Optimization of Camptothecin Biosynthesis by Aspergillus terreus and Penicillum sclerotigenum.

Document Type : Original Article

Authors

1 Department of botany, Faculty of Science, Mansoura University, 35516 Mansoura City, Egypt1

2 Department of botany and Microbiology, Faculty of Science, Zagazig University, 44519 Zagazig, Egypt

Abstract

Camptothecin (CPT) is a cytotoxic quinoline alkaloid that inhibits the DNA enzyme topoisomerase I (topo I). Endophytic and filamentus fungal isolates recovered from various medicinal plants were screened for their CPT biosynthetic potency. Among these isolates, Aspergillus terreus and Penicillum sclerotigenum recovered from Cinnamomum camphora and Ficus elastica respectively were reported as potent camptothecin producers (89.4 mg/l and 80.2mg/l). The chemical identity of A. terreus and P. sclerotigenum extracted CPT was verified by Thin Layer Chromatography (TLC) and Ultra Violet Spectroscopy Analyses (UV). Upon using the Blackett-Burman design to optimize their nutritional requirements by the selected fungal isolates, their yield of camptothecin was increased by approximately 7 folds, revealing the essentiality of some carbon, nitrogen sources, and growth elicitors for the biosynthesis of camptothecin.

Keywords

Mansoura Journal of Biology
Volume 63, Issue 2
June 2023
Pages 12-22

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