Gene expression of Acetyl Co-A carboxylase A (accA) in Synechocystis salina under nitrogen starvation and low salinity stress

Authors

1 Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt

2 Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt.

3 Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt

Abstract

Cyanobacteria are potential sources of valuable products such as proteins, lipids, pigments, vitamins and polysaccharides. lipids are considered one of the primary active metabolites in the cyanobacterium cell. Therefore, the usage of the unicellular cyanobacterium Synechocystis salina as a sustainable biological source for the accumulation of lipids is the main goal of the current research. Environmental stress such as low salinity (25 ppt sea salt) increased the lipid content of S.salina to 13.883% DW compared with the control. Nitrogen deficiency also elevated the lipid content of S.salina to 11.87% DW compared with control at adequate N supply. Acetyl Co-A carboxylase A (accA) catalyzes the first step in fatty acid biosynthesis; therefore, the monitoring of its transcription level is crucial to understand the effect of different stressors on lipid biosynthesis. The expression level of accA gene in S.salina was upregulated to 8.38 and 35 folds under nitrate depletion and 25 ppt sea salt respectively.

Keywords

Mansoura Journal of Biology
Volume 63, Issue 2
June 2023
Pages 53-58

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