Detection of Chlorhexidine Resistant Genes in Methicillin Resistant Staphylococcus aureus in Mansoura University Hospitals

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Mansoura University, Egypt

2 Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) is the main reason for severe human infections in hospital settings as well as in the community. Objective: This study aims to detect chlorhexidine resistance of MRSA by phenotypic and genotypic methods. Results: Fifty MRSA strains were isolated from different hospitals of Mansoura University over a period of one year from December 2021 to November 2022.The antimicrobial susceptibility test was performed by the disk diffusion method. The highest resistance was shown to Penicillin (100%) and Ceftazidime (100%) and the highest susceptibility was shown to Vancomycin (72%).The minimum inhibitory concentration (MIC) towards chlorhexidine (CHG) was determined by the two-fold microdilution broth method. Reduced susceptibility to CHG at MIC ≥4 mg/L was detected in 48% of isolates (24/50). Polymerase Chain Reaction was used to detect qac genes (norA, qacA/B1, qacA/B2 and smr). The most frequently detected genes were smr in 24 isolates (100%), norA in 12 isolates (50%), and qacA/B2 in 11 isolates (45.8%), with a low prevalence of qacA/B1 (1 isolate; 4.16%).

Keywords

Mansoura Journal of Biology
Volume 64, Issue 3
September 2023
Pages 12-18

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