Detection of Chlorhexidine Resistant Genes in Methicillin Resistant Staphylococcus aureus in Mansoura University Hospitals

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Mansoura University, Egypt

2 Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt

10.21608/mjb.2023.449531

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) is the main reason for severe human infections in hospital settings as well as in the community. Objective: This study aims to detect chlorhexidine resistance of MRSA by phenotypic and genotypic methods. Results: Fifty MRSA strains were isolated from different hospitals of Mansoura University over a period of one year from December 2021 to November 2022.The antimicrobial susceptibility test was performed by the disk diffusion method. The highest resistance was shown to Penicillin (100%) and Ceftazidime (100%) and the highest susceptibility was shown to Vancomycin (72%).The minimum inhibitory concentration (MIC) towards chlorhexidine (CHG) was determined by the two-fold microdilution broth method. Reduced susceptibility to CHG at MIC ≥4 mg/L was detected in 48% of isolates (24/50). Polymerase Chain Reaction was used to detect qac genes (norA, qacA/B1, qacA/B2 and smr). The most frequently detected genes were smr in 24 isolates (100%), norA in 12 isolates (50%), and qacA/B2 in 11 isolates (45.8%), with a low prevalence of qacA/B1 (1 isolate; 4.16%).

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Mansoura Journal of Biology
Volume 64, Issue 3
September 2023
Pages 12-18

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