Optimization of iron oxide nanoparticles biosynthesis: a selection of the efficient bactericidal and/or bacteriostatic type against pathogenic bacteria

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Sciences, University of Mansoura, Egypt,

2 Division of biophysics, Department of physics, faculty of science, University of Mansoura, Egyp

3 Department of agricultural microbiology, national research center, Egypt.

10.21608/mjb.2023.449536

Abstract

The recent widespread infectious diseases associated with pathogenic bacteria, i.e., Staphylococcus aureus, Salmonella typhi, Escherichia coli and Klebsiella pneumonia are a serious health issue. Regarding the potentiality of magnetotactic bacteria,  Pseudomonas aeruginosa kb1 (KT962901) in biosynthesis of nanoparticles, this research study was designed to improve the synthesis conditions of iron oxide nanoparticles (IONPs) and subsequently evaluate the efficacy of each factor in controlling the growth of specific pathogenic bacteria. In the research methodology, the different factors were investigated to optimize the biosynthesis process, i.e.,  medium type, aeration conditions, concentration, salt type and point of salt addition. The shape and structure of the synthesized IONPs were confirmed using Fourier transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy Dispersive X-ray diffraction (EDX) analysis, Zeta potential and Dynamic light scattering (DLS). The well diffusion method was used to assess the efficiency of IONPs in suppressing the growth of target bacteria. The study's findings showed that clear zones against two or three of the tested bacteria were present in 13 of the 36 tested samples utilized in the initial screening to treat the pathogenic bacteria. There are just two samples (trials no. 6 and 13) that have demonstrated outstanding antibacterial action against both Gram-negative and Gram-positive bacteria after the second screening using higher concentrations of the 13 patches. In conclusion, optimizing the process of iron oxide nanoparticles biosynthesis by Pseudomonas aeruginosa kb1 may provide a more effective method in controlling some pathogenic bacteria.

Keywords

Mansoura Journal of Biology
Volume 64, Issue 3
September 2023
Pages 19-31

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