Investigation of multifunctional potential of Lactobacillus acidophilus-DSM20079 in wound infection model

Document Type : Original Article

Authors

1 Botany Department, Faculty of Science, Mansoura University.

2 Botany Department, Faculty of Science, Tanta University

3 The Regional Center for Mycology and Biotechnology, Al-Azhar University, Nasr City, Cairo 11787, Egypt

10.21608/mjb.2023.449543

Abstract

Bacterial colonization and infection remain the major causes of delayed healing following burns. Topical treatment is necessary to reduce the incidence of burn wound infection. However, this treatment produces adverse reactions and side-effects.                                                                                                                                          Lactic acid bacteria (LAB), are very important organisms because, they have no harmful effects, and are available in fermented food. The study was aimed to evaluate the potential applications of Lactobacillus acidophilus DSM20079 as the anti-inflammatory and antimicrobial against several pathogenic organisms as well as  its effect on wound healing., we performed this exploratory study to establish the effectiveness of bacteriotherapy with topical application of the bacteria Lactobacillus acidophilus DSM20079 to provide an alternative method for burn treatment using chloramphenicol as microbicidal agent as a reference drug. These bacteria would compete with other bacteria that are wound pathogens and would modify the wound environment and promote tissue repair. L. acidophilus-DSM20079 filtrate and lyophilized cells demonstrated significant antimicrobial properties. The highest antibacterial effects were detected against Staphylococcus aureus then Escherichia coli and Micrococcus luteus, respectively. The in vitro anti-inflammatory action was tested using lipoxygenase enzymatic inhibition assay where Lactobacillus acidophilus DSM20079 lyophilized cells solution showed good inhibition of lipoxygenase action with IC50 value 13.3±2.1 µg/ml, while IC50 =12.7±1.9µg/ml for Lactobacillus acidophilus DSM20079 filtrate versus standard dug IC50 value of 1.3±0.7 µg/ml. Agar well diffusion assay was also performed to test the antibacterial action against 8 bacterial strains. Moreover, crude metabolites of Lactobacillus acidophilus DSM20079 had a significant antimicrobial activity among different bacterial strains. The highest inhibition zone value (20.2±1.8 mm) of metabolites of Lactobacillus acidophilus DSM20079 was observed for the S. aureus strain. 

Keywords

Mansoura Journal of Biology
Volume 64, Issue 3
September 2023
Pages 53-65

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