Antimicrobial activity of biogenic silver nanoparticles synthesized with the aid of Citrus peel

Document Type : Original Article

Authors

1 Botany Department, Faculty of Science, Mansoura University, 35516 Mansoura, Egypt

2 Botany and Microbiology Department, Faculty of Science, Arish University, 45511 Arish, Egypt

3 Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt

10.21608/mjb.2023.449550

Abstract

Silver nanoparticles (AgNPs) have gained considerable attention as antimicrobial agents. The present study aimed at synthesizing AgNPs using navel orange (Citrus sinensis L.) fruit peel. Citrus peel (CP) was extracted in water using different methods. The ability of CP extracts to mediate AgNPs synthesis from AgNO3 was monitored by visual observation, ultraviolet-visible spectral analysis and electron microscopy. The change in reaction mixture color from yellow to brown primarily indicated AgNPs formation. In addition, absorption bands at about 450 nm confirmed the formation of AgNPs. Transmission electron microscopy revealed that AgNPs were almost spherical with average size < 30 nm. Antimicrobial activity of AgNPs was tested against Bacillus subtilis and Staphylococcus epidermidis using disk diffusion method. Clear zones were formed with inhibition diameter of 44.1 mm for B. subtilis and 35.2 mm for S. epidermidis. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs were found to be 0.3 mg/ml for the two microbes. AgNPs could inhibit respiratory chain dehydrogenases and induce cellular sugar and protein leakage as well as lipid peroxidation of the two microbes. However, the effect of AgNPs on the activity of dehydrogenases and lipid peroxidation of B. subtilis was more pronounced than that on S. epidermidis. The reverse was recorded for the change in cellular sugar and protein leakage where the effect of AgNPs was more pronounced on S. epidermidis. Therefore, biogenic AgNPs synthesized with the aid of CP extract can be recommended as potent antimicrobial agent against B. subtilis and S. epidermidis.

Keywords

Mansoura Journal of Biology
Volume 65, Issue 4
December 2023
Pages 10-16

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