Document Type : Original Article
Authors
1
Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt.
2
Environmental Virology Laboratory, Water Pollution Research Department, Environment and Climate Change Research Institute, National Research Center, Dokki 12622, Giza, Egypt.
3
Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, Dokki 12622, Giza, Egypt.
4
Microbial Biotechnology Department, Biotechnology Research Institute, National Research Centre, Dokki 12622, Giza, Egypt
Abstract
In this study, Aspergillus flavus var. columnaris and Penicillium goetzii were used for bio-synthesizing of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) respectively, using eco-friendly and cost-effective methods. After biosynthesizing of nanoparticles, it was characterized by UV–Visible Spectroscopy, Fourier-Transform Infrared (FTIR), Transmission Electron Microscopy (TEM), Selected Area Electron Diffraction (SAED) and Dynamic Light Scattering analysis (DLS). Bio-synthesized AgNPs and AuNPs were tested as antibacterial against gram positive and gram negative bacterial strains: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella sp., Klebsiella sp. and Proteus mirabilis, as antifungal against Aspergillus niger and Candida albicans and as antiviral against human adenovirus serotype 5 (HAdV-5). Furthermore Bio-synthesized AgNPs and AuNPs were tested as anticancer against HepG2 hepatocarcinoma cells. Maximum absorbance at 540 nm and 440 nm was obtained using UV–Visible absorption spectra for reaction mixtures of AuNPs and AgNPs respectively. DLS measurements results confirmed UV–Visible absorption results, that the highest intensity of the AgNPs and AuNPs were 84 nm and 73.9 nm, respectively. Some functional groups were identified using FTIR measurements (e.g. Alcohol, Alkane, cyclopentanone, carbon dioxide, Amine, conjugated alkene,…etc.) detected in Penicillium goetzii and Aspergillus flavus var. columnaris cultures filtrates that could be responsible for production of AgNPs and AuNPs. Particles size and morphologies of AgNPs and AuNPs were confirmed by the SAED pattern analysis and TEM. Green synthesized AuNPs and AgNPs showed good anticancer and antimicrobial activities.
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