histological and immunohistochemical studies of the effect germinating fenugreek (trigonella arabica) and barley (hordeum vulgare) grains on spinal cord of 21-day-old offspring rats maternally fed on high fat diet.

Document Type : Original Article

Authors

1 Department of Zoology, Faculty of Science, Mansoura University,Mansoura, Egypt

2 Fellow of Comparative Anatomy and Embryology, Department of Zoology, Children’s hospital, Mansoura University, Mansoura, Egypt.

Abstract

This research studies the effect of feeding on high fat diet (HFD) on the structure and function of the rat offspring spinal cord and the good effect of feeding on germinating fenugreek (Trigonella arabica) and barley (Hordeum vulgare) grains. After mating, pregnant rats were divided four groups (n=10); control, HFD (20% soft animal fat with standard nutritional diet), fenugreek & HFD (20% soft animal fat with germinated fenugreek grains) and barley & HFD (20% soft animal fat with germinated barley grains) were experimentally tested using Hematoxylin and Eosin stain to detect their histological changes and immunohistochemical analysis to detect tissue damage using P53 and synaptophysin. HFD group offspring showed adverse effects on spinal cord. HFD induced inflammatory cells and degenerated spinal cord with accumulated fat droplets and atrophied nucleus. Phytochemical analysis was performed on extracted samples from Fenugreek (Trigonella arabica) and Barley (Hordeum vulgare) grains and revealed high antioxidant content. This explained why dietary supplementation of either germinating barley or fenugreek grains showed good effects and improvement in the structure and function of spinal cord.

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Mansoura Journal of Biology
Volume 67, Issue 6
December 2023
Pages 22-27

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