Optimizing Cellulase Production from a thermo stable bacterium Rhodthermus marinus

Document Type : Original Article

Authors

Department of Botany, Faculty of Science, Mansoura University, 35516, Egypt

Abstract

The high demand of a cheap and sustainable energy source are necessity for all nation all over the world. Cellulose fulfills these conditions and it is the most abundant energy source on earth. Conversion of cellulose into renewable biofuels such as ethanol or butanol requires digestion by cellulases, which are abundant in microbial world. Thermotolerant bacteria are good and promising producers of this enzyme. In this study, we optimized the growth condition of R. marinus to produce the highest activity of this enzyme. The Centeral Composite Design was used to optimize the physical factors (pH, temperature, and incubation period), and Placket-Burman experimental design was used to determine the best nitrogen and minerals sources. The enzyme activity was determined by the reducing sugar method, which showed that manganese sulphate (0.01g/L) was the best. While, yeast extract was the best for growth of the tested bacterium and the malt extract produced the highest amount of enzyme. In conclusion, the study showed that CMC, malt extract and yeast extract in presence of manganese sulphate R. marinus produced the highest concentration of thermotolerant cellulase needed for conversion of cellulose into biofuel.

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Mansoura Journal of Biology
Volume 56, Issue 3
September 2022
Pages 55-66

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