Histological and immunohistochemical studies of the effect of nano-zinc on harderian gland of mother rats and their offspring treated with lipo polysaccharides.

Document Type : Original Article

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Department of Zoology, Faculty of Science, MansouraUniversity,Mansoura, Egypt

Abstract

The present study focused on the therapeutic effect of zinc oxide nanoparticles (ZnO NPs) on the Harderian gland of mother rats and their offspring at 21 day-old affected by lipopolysaccharides. Eight male and forty virgin female rats (Rattus norvegicus) were used in this study. After mating, pregnant rats divided into four groups; control, ZnO NPs-treated, lipopolysaccharides (LPS)-treated, and LPS & ZnO NPs-treated groups. ZnO NPs with or without LPS were injected four times on the 14th, 17th, and 20th days of gestation, plus a dose after birth. LPS-treated group was injected two times on the 7th and 9th days of gestation. The mothers and their 21-day-old offspring were sacrificed and the Harderian glands were removed for histological and Proliferating Cell Nuclear Antigen (PCNA) immunohistochemistry examination. The therapeutic role of ZnO NPs in LPS and ZnO NPs-treated rats appeared in the histological improvement and reducing immunostaining of the mothers and offspring Harderian glands affected by LPS.

Keywords

Mansoura Journal of Biology
Volume 57, Issue 4
December 2022
Pages 57-63

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