Protective role of MGN-3/Biobran against dimethylhydrazine- induced toxicity in rats

Document Type : Original Article

Authors

1 Department of Zoology, Faculty of Science, Mansoura University,

2 Department of Zoology Faculty of Science, Tanta University

3 Department of Zoology, Faculty of Science, Mansoura University

Abstract

Dimethylhydrazine (DMH) is a potent colonic and hepatic carcinogen that is metabolized into oxyradicals, causing liver injury and DNA mutations. Arabinoxylan rice bran (MGN-3/Biobran) is a well-documented natural product that possesses anti-inflammatory, antioxidant and anti-carcinogenic properties. The main objective of the study is to determine the protective potential of MGN-3/Biobran against DMH-induced toxicity in vital organs of rats. The study was performed using Sprague Dawley rats allocated into four groups. The first group is the normal untreated control, 2nd group received MGN-3/Biobran only, 3rd group received subcutaneous injection of DMH-2HCL once per week for 4 consecutive weeks. Rats in the 4th group were pretreated with MGN-3/Biobran, 2 weeks prior to the injection with DMH-2HCL. Biobran treatment was continued throughout the experimental period. After 18 weeks of experimental period, the organs (liver, spleen and kidney) were excised and the blood samples were collected then the sera were separated. Serum was used for the determination of liver and kidney function biomarkers and lipid profile parameters. The results showed that DMH induced markedly decreased organ weights, increased total bilirubin, decreased albumin and total protein content, increased serum concentrations of urea, creatinine, cholesterol and triacylglycerol. Protective effects of Biobran were observed by maintaining normal liver, spleen and kidney weights, ameliorating all biochemical parameters within normal values. It can be concluded that MGN-3/Biobran may be a promising chemopreventive agent against DMH toxicity.

Keywords

Mansoura Journal of Biology
Volume 59, Issue 6
December 2022
Pages 26-33

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