The possible protective role of Graviola Leaf Extract against CCl4 hepatotoxicity in Male Albino Rats

Document Type : Original Article

Authors

Physiology Division, Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt

10.21608/mjb.2021.460001

Abstract

The objective of the current study is to establish the protective effect of Graviola Leaf Extract (GLE) against Carbon tetrachloride (CCl4) hepatotoxicity in male albino rats. Rats were divided randomly into four groups, each one contains 10 rats as follows:  Group 1(control): rats received distilled water; group 2 (GLE): rats received GLE (200 mg / kg bw) through oral administration daily for one month; group 3 (CCl4): rats received CCl4 (1ml / kg bw) intraperitoneal administration as single dose at the last day of the experiment; group 4 (GLE+ CCl4): rats received GLE and CCl4 as group 2 and 3. The obtained results showed  significant increase in hepatic enzymes (AST, ALT) and TB in serum and also showed significant increase in oxidative stress markers (MDA) as well as significant decrease in serum Alb and in antioxidant parameters (GSH, SOD, CAT) in CCl4 treated group, it also causes significant increase in proinflammatory cytokines level (IL- 1β, TNF-α) and in apoptotic markers (Bax, caspase-3) and caused a significant decrease in anti-apoptotic protein (Bcl‑2), as well as it caused DNA damage and significant increase in (Tail moment, Tail length, Tail DNA), Histology examination also showed hepatic tissue damage. On the other hand, GLE administration significantly improved the deviation resulting from CCl4 in nearly all parameters. In conclusion GLE is able to reduce the hepatic damage caused by CCl4 and GLE is recommended to be taken as a protective agent against hepatotoxicity

Keywords

Mansoura Journal of Biology
Volume 53, Issue 4
December 2021
Pages 1-9

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