A novel proteolytic fungus, statistical screening of medium components for amino acids production

Document Type : Original Article

Authors

1 Assistant Researcher in Microbiology Dep Agricultural Research Center – Giza, Egypt

2 Professor of Microbiology, Botany Dep Faculty of Science, Mansoura Univ

3 Assistant Professor of Radiation Microbiology Atomic Energy Authority (AEA), Egypt

4 Microbiology Dep Agricultural Research Center – Giza, Egypt

10.21608/mjb.2021.460020

Abstract

Soybean as a cheap and aliquot agricultural by-products have the ability in applying the entire SSF process. Evaluation of the effective conditions for amino acid production, the statistical experimental design of Pl ackett–Burman was applied for the improvement of protease yield by Aspergillus flavus using soybean residuals as raw materials. Plackett–Burman design had been applied to value the effects of six variables: independent variable soybean residuals as a substrate, spore suspension, the incubation period, pH, MgSO4.7H2O, (NH4)2SO4 at high concentration for all factors as 13 day, 6 mL/g and 2.2 mL/g respectively. The key factors influencing protease production and production of free amino acids were identified as incubation period, the concentration of KH2PO4, and concentration of (NH4)2SO4. The statistical experimental design was proven as an effective pathway to obtain optimum parameters for amino acids production 

Keywords

Mansoura Journal of Biology
Volume 52, Issue 3
October 2021
Pages 31-38

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