MICROBIAL BIOTRANSFORMATION OF BIOACTIVE NATURAL PRODUCTS FOR DRUG DISCOVERY

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Mansoura University, 35516 Mansoura City, Egypt

2 Plant pathology Research institute -Agriculture Research center-Giza-Egypt

10.21608/mjb.2021.460021

Abstract

Microbial biotransformation is used instead of chemical synthesis or mammalian models to study drug safety where many fungi possess cytochrome p450 as in mammalian. Biotransformation is a process used to convert one molecule to other by whole-cell or isolated enzymes. About 40 fungi were isolated from different soil and plant residues. All these isolates were identified morphologically and used to study their potential to transform alkaloid piperine. Among these fungi, four fungi namely Aspergillus herbariourm, Aspergillus ochraceous, Cladosporium sp, and Stachybotrius sp showed the ability to metabolize piperine. Among the four fungi, only Aspergillus herbariourm was used in the preparative scale fermentation procedure to give five metabolites identified by GC/MS analyses technique. These metabolites were identified as hexyl(methylidyne)ammonium, dimethyl phenyl ethyltethylidyl pyrrolidine, (4E)-hepta-1.2.4.6-tetraeane methyl laurate, 6,7diazabicyclo[3,2,2] and non a-3,6-diene,2-methylene.

Keywords

Mansoura Journal of Biology
Volume 52, Issue 3
October 2021
Pages 39-44

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