Molecular And Biological Exploring Studies On Mycovirus Of Egyptian isolate Of Trichoderma hamatum

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Mansoura University, 35516 Mansoura City, Egypt

2 Botany and Microbiology Department, Faculty of Science, Damietta University, Damietta, Egypt

10.21608/mjb.2021.460044

Abstract

Trichoderma spp. are used extensively in agriculture as biological control agents to prevent soil-borne plant diseases. Seventeen fungal species were isolated from different sites of four governorates Dakahlia, Al-Sharqiya, kafr-Elsheikh and Damietta. The count of each fungal isolate was generally affected by physicochemical features of collected soils during the studies. The isolated fungal strains were morphologically classified into three classes namely; Zygomycetes, Ascomycetes and Hyphomycetes. Hyphomycetes was the most predominant class and was represented by three genera namely, Alternaria, Fusarium and Trichoderma. Ascomycetes was the second dominant class and was represented by two genera namely Aspergillus and Penicillium. Zygomycetes was represented by only one genera namely Rhizopus. Among all fungal isolates of this study, T. hamatum contained a single double-stranded RNA (dsRNA) segment of approximately 3 kb, which is typical of mycoviruses. We designated this mycovirus as Trichoderma hamatum mycovirus 1(ThaMV1). Transmission electron microscopy of (ThaMV1) revealed the presence of putative virus-like particles (VLPs) with an isometric structure approximately of 50 nm in diameter. This study represents the first report of isolation of a new unclassified mycovirus infecting T. hamatum

Keywords

Mansoura Journal of Biology
Volume 51, Issue 2
June 2021
Pages 1-7

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