Biological control of pathogenic bacteria by plant growth promoting rhizobacteria Bacillus amyloliquefaciens MG214652

Document Type : Original Article

Authors

Botany Department, Faculty of Science, Mansoura University

10.21608/mjb.2021.460285

Abstract

Employing plant growth promoting rhizobacteria (PGPR) as biocontrol agents is of considerable interest. Some PGPR has the ability to produce one or more antibiotics that act as antimicrobial agent against pathogens. In this study, Bacillus MAP3 which was isolated from root nodules of Phaseolus vulgaris is evaluated as a biocontrol agent. The isolate MAP3 appeared milky white, opaque, irregular in shape, smooth and 2-4 mm in diameter after incubation at 37°C for 24 h and was found to be a Gram-positive spore-forming bacilli. The isolate MAP3 was tested for its antimicrobial activity against 9 pathogens. The antimicrobial activity was great against Erwinia carotovora and Bacillus subtilis (inhibition zone more than 25 mm), moderate against Candida albicans (inhibition zone 16 mm), weak against E. coli, Klebsiella spp, Staphylococcus epidermidis and Pseudomonas aeruginosa (inhibition zones were 11, 12, 10, 10 mm respectively) and there was no effect against Shigella spp. and Proteus vulgaris. The metabolites of this isolate showed antimicrobial activity when tested against Erwinia carotovora with an inhibition zone 20 mm. The results obtained put this isolate as a promising biocontrol agent that would be used against further pathogens.

Keywords

Mansoura Journal of Biology
Volume 50, Issue 1
March 2021
Pages 53-59

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