Histomorphometric study of the protective role of purslane seed extract on bone development of rat fetuses maternally treated with titanium dioxide nanoparticles.

Document Type : Research Article

Authors

1 Zoology Department, Faculty of Science (Girls),Al-Azhar University, Cairo, Egypt.

2 Histology and cell biology Department - Faculty of Medicine (Girls), Al-Azhar University, Cairo, Egypt.

3 1Zoology Department, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.

10.21608/mjb.2020.460393

Abstract

Titanium dioxide nanoparticles (TiO2NPs) are widely used in many commercial products. Early developmental stages of rats are sensitive to TiO2NPs especially during bone development. Purslane (P) seeds are used as natural source of antioxidants. The present study was designed to evaluate the possible protective role of P seed extract on the structure of femur tissue of 20-day old fetuses maternally treated with TiO2NPs during pregnancy. Thirty-two pregnant rats were randomly divided into four groups. Group (C): untreated control. Group (P): rats were treated with 10 ml/kg/day P extract. Group (T): rats received 0.5mg/kg/day TiO2NPs. Group (P+T): rats received P extract, followed by TiO2NPs at the same previous dose. All doses were orally administered from the 6th to the 15th day and rats were sacrificed on the 20th day of gestation. The fetuses were exposed and fetal hind limbs were cut and fixed in formol then decalcified and stained for histological study. Titanium dioxide nanoparticles induced alterations in the fetal femur tissue as incomplete chondrification of mesenchymal cells, few cartilaginous cells in resting zone, decreased width of proliferation zone, decreased size of chondrocytes in the hypertrophic zone as well as degenerated chondrocytes in calcification zone. However, P improved the destructive changes caused by TiO2NPs. The length and width of femur and tibia bones of P treated group as well as distribution of collagen fibers was improved. Therefore, it is recommended to be very careful while dealing with nanomaterials. It is preferred for eating leafy green vegetables rich in antioxidants during pregnancy.

Keywords

Mansoura Journal of Biology
Volume 49, Issue 4
December 2020
Pages 69-79

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