Conventional and Molecular Techniques for Diagnosis of Bacterial Pneumonia

Document Type : Research Article

Authors

1 Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

2 Department of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

3 Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt

10.21608/mjb.2020.460717

Abstract

This study aimed to detect bacterial causes of pneumonia and antibiotics that can be used for the treatment. In addition to detect the prevalence of atypical pneumonia caused by Mycoplasma pneumoniae, Legionella pneumophila and Chlamydia pneumoniae among strains isolated from patients in different Mansoura University Hospitals, Mansoura City, Egypt, due to its importance in treatment of human infections. Fifty five clinical isolates from 88 samples were detected and identified by morphological and biochemical methods. The result of isolation showed that Citrobacter diversus (41.4%) was the most common pathogen detected in adults and Pseudomonas aeruginosa (42%) was the most common pathogen detected in children 42%. Their antimicrobial susceptibility to 17 antimicrobial agents from 6 antimicrobial categories (aminoglycosides, carbapenems, cephalosporins, fluoroquinolones, penicillins, glycopeptides) was determined by disk diffusion method, according to recommendation of Clinical and Laboratory Standards Institute. The highest resistance was shown to cefaclor and cefuroxime (98%) and the highest susceptibility was shown to imipenem (70%). The result of the study showed that no atypical bacteria were detected in sputum and endotracheal aspirate (ETA) of patients with pneumonia using multiplex PCR. 

Keywords

Mansoura Journal of Biology
Volume 48, Issue 3
September 2020
Pages 39-44

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