Document Type : Original Article
Authors
1
Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt
2
Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), Alexandria 21934, Egypt.
3
Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Abstract
Collagen nanoparticles (collagen-NPs) have several advantages over other natural and synthetic polymeric NPs, including suitable biocompatibility and biodegradability, low antigenicity, a high contact surface, and reduced toxicity. Collagen-NPs appropriate for use in the field of biomedicine in skin wound healing, tissue engineering, drug delivery, in bone grafting, and healing as they promote optimal bone regrowth and for treatment of the damaged nerve tissues and nerve regeneration. Eight strains were assessed for their abilities for the biosynthesis of collagen-NPs using their cell-free supernatant. After incubation, white turbidity appeared as the result of collagen-NPs formation. The most potent strains for maximum biosynthesis of collagen-NPs were identified as Streptomyces xinghaiensis strain NEAA-1 and Streptomyces plicatus strain NEAA-3. These Streptomyces strains were tested for collagen-NPs biosynthesis under different conditions, such as concentrations of collagen and strains cell-free supernatant, temperature, initial pH, and incubation time. The maximum biosynthesis of the collagen-NPs was 4.13 mg/mL and 5.16 mg/mL, respectively, at 35 ºC and 40 ºC for Streptomyces xinghaiensis and Streptomyces plicatus. The maximum biosynthesis of the collagen-NPs was 5.24 mg/mL and 6.33 mg/mL, respectively, at 7 pH for Streptomyces xinghaiensis and Streptomyces plicatus. The maximum biosynthesis of the collagen-NPs was 6.51 mg/mL and 7.42 mg/mL after 48 h for Streptomyces xinghaiensis and Streptomyces plicatus, respectively. The biosynthesized collagen-NPs showed 96% anti-hemolytic activity.
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